RAGE protein levels are decreased in the lungs after injury.

<p>Aliquots of 10 µg of protein from membrane or soluble protein fractions from lung homogenates were assayed for RAGE expression by western blot analysis of RAGE and β-actin. Net intensity of RAGE expression was normalized to β-actin. Membrane RAGE expression was significantly decreased after both LPS and <i>E. coli</i> challenge (A). Soluble RAGE expression was significantly decreased after <i>E. coli</i> injury, and markedly, but not significantly decreased after LPS injury (p = 0.057 for LPS treatment). Normalized net intensities were analyzed by a Mann-Whitney test. (* p<0.05, <i>n of 4 mice per group</i>).