Foxd1DTA mutants have thickened and widened nephron progenitor caps.

<p>A–B: E13.5 Six2 staining reveals that mutant kidneys (B) have thicker nephron progenitors and widened progenitor caps (arrow) compared to controls (A). C–F: At E18.5, Six2 staining shows the nephron progenitor caps were thicker in the mutants (D) compared to controls (C). It is also apparent that the nephron progenitor caps experience a widening, with some mutant caps being almost twice the size (D) of the control caps (C). E–H: Amphiphysin staining reveals similar nephron progenitor thickening and disorganization of the nephron progenitors that remains apparent at E18.5 (H). I–L: Pax2 staining of the nephron progenitors confirms nephron progenitor thickening at E13.5 in mutants (J) compared to controls (I). At E18.5 the large and unorganized nephron progenitor caps are apparent in mutants (L). Scale bar = A–B:100 µm; C–F:25 µm.