Loss of <i>Cdc42</i> phenocopies <i>Yap^CM−/−</i> phenotype.

<p>(A,B) Macroscopic view of the urogenital system from wild-type and <i>Cdc42^CM−/−</i>kidneys at P0. Note the reduction in kidney bladder size in mutant animals. (C–F) PAS staining (P0) from wild-type and <i>Cdc42^CM−/−</i> animals showing smaller papilla (arrows), dramatic reduction of both CM-derived epithelial structures and glomeruli in the mutant. (G–J) Sections of P0 kidneys using late nephron-specific markers confirms the abnormal glomeruli and proximal tubules formation in <i>Cdc42</i> mutant kidneys. Glomeruli (Podocin, G,H). Proximal tubules (LTL, I,J). (K,L) NCAM staining (E15.5) reveals dramatic reduction in the number of CM-derived structures (arrowheads) in mutants compared to wild-type. k: kidney; b: bladder; g: glomeruli; pt: proximal tubule. Scale bars represent 1 mm (A–D), 200 µm (E–L).