Simulation of myosin inhibition.

<p>The same simulation as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087638#pone-0087638-g004" target="_blank">Fig. 4</a>, but with a myosin contractility of 0.225 pN/µm² instead of 5.555 pN/µm². (A) Position of the leading edge (black) and the gel boundary (blue). (B) Velocities of the leading edge (black) and the gel boundary (light blue) and retrograde flow velocity (red). Comparison with <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087638#pone-0087638-g004" target="_blank">Fig. 4</a> shows that the measured increase in period (C) is reproduced by our simulation. Filament densities, filament lengths and SR depth show basically the same behavior as without myosin inhibition, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087638#pone-0087638-g004" target="_blank">Fig. 4 C, D</a>. (C) The measured velocity map from Burnette et al. (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087638#pone-0087638-g004" target="_blank">Fig. 4</a> from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087638#pone.0087638-Burnette1" target="_blank">[20]</a>) shows that the period of protrusion cycles increases when myosin is inhibited by application of Blebbistatin. Published with permission from Nature Cell Biology.