Data from: The selective myosin II inhibitor blebbistatin reversibly eliminates gastrovascular flow and stolon tip pulsations in the colonial hydroid Podocoryna carnea

Blebbistatin reversibly disrupted both stolon tip pulsations and gastrovascular flow in the colonial hydroid Podocoryna carnea. Epithelial longitudinal muscles of polyps were unaffected by blebbistatin, as polyps contracted when challenged with a pulse of KCl. Latrunculin B, which sequesters G actin preventing F actin assembly, caused stolons to retract, exposing focal adhesions where the tip epithelial cells adhere to the substratum. These results are consistent with earlier suggestions that non-muscle myosin II provides the motive force for stolon tip pulsations and further suggest that tip oscillations are functionally coupled to hydrorhizal axial muscle contraction.Intact replicate 1_prior to reagentTiff files from time-lapse movie acquired during blebbistatin treatment of intact stolon tipIntact replicate 1_after reagentIntact replicate 1_recoverytiff images from period of film following clearance of blebbistatinIntact replicate 2_prior to reagentIntact replicate 2_after reagentIntact replicate 2_recoveryIntact replicate 3_prior to reagentIntact replicate 3_after reagentIntact replicate 3_recoveryintact replicate 4 prior to reagentintact replicate 4 after reagentintact replicate 4 recoveryIntact replicate 5 prior to reagentIntact replicate 5 after reagentIntact replicate 5 recoveryIsolated replicate 2 prior to reagentIsolated replicate 2 after reagent addedIsolated replicate 2 recoveryIsolated replicate 3 prior to reagentIsolated replicate 3 after reagentIsolated replicate 3 recoveryLatrunculin replicate 1 prior to reagentLatrunculin replicate 1 after reagentLatrunculin replicate 1 recoveryLatrunculin rep 2_fig 7_pt 1Latrunculin rep 2_fig 7_pt 2Latrunculin replicate 3 prior to reagentLatrunculin replicate 3 after reagentLatrunculin replicate 3 recoveryIsolated rep 1_fig 6_pt 1aIsolated rep 1_fig 6_pt 1bIsolated rep 1_fig 6_pt 2aIsolated rep 1_fig 6_pt 2bIsolated rep 1_fig 6_pt 2cIsolated rep 1_fig 6_pt 2dIntact rep 6_fig 5_part 1aIntact rep 6_fig 5_part 1bIntact rep 6_fig 5_part 1cIntact rep 6_fig 5_part 1dIntact_rep 6_fig 5_pt 2aIntact_rep 6_fig 5_pt 2bIntact_rep 6_fig 5_pt 2cIntact_rep 6_fig 5_pt 2dIntact_rep 6_fig 5_pt 2eIntact_rep 6_fig 5_pt 2fIntact_rep 6_fig 5_pt 2gIntact_rep 6_fig 5_pt 2hIntact_rep 6_fig 5_pt 2iIntact_rep 6_fig 5_pt 2jFigure 5 time and lengthFigure 6 time and lengthFigure 7 time and lengthREAD MEKey to all other file