Blebbistatin effect on ramp shortening critical points in experiment and model simulation.

<p>(A) Experimentally measured P₁ for different ramp velocities. Solid line: pCa²⁺ 4.5 with blebbistatin, dotted and dashed line: pCa²⁺ 4.5 and pCa²⁺ 6 without blebbistatin, respectively. (B) P₁ detected in simulation for different ramp velocities. Solid line: blebbistatin inhibition modeled by lowering of myosin actin tight binding energy by ΔE = 0.35k_BT. Dashed line: no blebbistatin inhibition. (C, D, E) Experimentally determined L₁, P₂, L₂, respectively; same conditions as in (A). (F, G, H) L₁, P₂, L₂ detected in simulated ramp shortening, respectively; same conditions as in (B). For simulation parameters see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0029356#pone.0029356.s005" target="_blank">Text S1</a>.