Increased frequency of PDX-1⁺ pancreatic progenitors emerging from the ducts in transplant microenvironments enriched in M2 macrophages.

<p>Tissue sections of E14–15.5 pancreatic epithelial grafts transplanted in BM-reconstituted Id1+/−Id3−/− mice (A–C) or WT controls (D–F), stained by two-color immuno-fluorescence for PDX-1 (green) and E-cadherin (red). Numerous PDX-1⁺ cells are comprised within or emerge from ductal structures in the grafts of BM-reconstituted Id1+/−Id3−/− mice (arrowheads). (G–H) Morphometric analysis of E-Cadherin⁺ grafts areas (G) and PDX-1⁺ cells counted within the grafts (H). Bars represent mean ± SEM of n = 4 experiments including a total of 8 grafts in Id1+/−Id3−/− and 6 grafts in WT mice. Scale bar = 60 µm; * <i>p</i>≤0.001.