Human BMSCs-PDX1 transplantation in chemically induced diabetic mice.

<p>Panel A shows different patterns of blood glucose levels in various groups of mice, temporary reversed (purple diamond, Rev), unrescued (black rectangle, Unrescued), control (white triangle) and STZ-induced diabetic (green cross, STZ) during study time period. Survival analysis (B) of diabetic mice injected with hBMSCs-PDX1 (purple line) shows 100% survival compared with the significant lifespan reduction of diabetic mice (blue line, <i>p<0.05</i>) at 6 weeks. Panel C shows weights in gram (g) of control mice (control), diabetic mice (STZ) and diabetic mice treated with hBMSCs-PDX1, indicating significant weight gains in control and hBMSCs-PDX1treated groups compared with one in the diabetic group during 6 week study period (day 42; purple bar) from the moment of stem cell transplantation (Day 0; blue bar). Immunoperoxidase staining analysis in pancreases shows stronger expression of insulin in control healthy mice (D) compared with ones in other groups of mice, STZ-induced diabetic (E), temporary reversed (F), and unrescued (G). Panel H shows coexpression of human β-2-microglobulin (green) and insulin (red) in the injured pancreas suggesting de novo differentiation of β-cells from injected hBMSCs-PDX1. Nuclei were counterstained with DAPI (Blue). Scale bar: 50 µm. <i>*p<0.05, **p<0.001.</i>