Frequency and proliferative potential of endocrine-committed progenitors in pancreatic transplants.

<p>(A–B) Tissue sections of E14–15.5 pancreatic epithelial grafts transplanted in BM-reconstituted Id1+/−Id3−/− mice (A) or WT controls (B), stained by two-color immuno-fluorescence for Insulin (green) and the proliferation marker PCNA (red). Dotted lines delineate boundaries of ductal structures. (C–E) Morphometric analysis of Insulin⁺ areas (C), proliferating Insulin⁺PCNA⁺ cells (D) and Glucagon⁺ areas (E), assessed in the grafts. Bars represent mean ± SEM of n = 3 experiments, including a total of 7 grafts in Id1+/−Id3−/− and 5 grafts in WT mice. Scale bar = 120 µm; *<i>p</i> = 0.01 and ** <i>p</i><0.002.