B20.4.1.1 Treatment During EAE Minimizes the Increase in Cell Numbers in Peripheral Lymph Nodes and Reduces Proliferative Responses in CD4⁺ T cells.

<p>Data are shown as mean ± SEM for wild type controls, untreated EAE, EAE+B20.4.1.1, EAE+K(1-3) and EAE+agents combined. Significance is relative to untreated EAE (ns = not significant, ***, P<0.05–0.001). A. Effect on total cell numbers (n = 3–5 samples for each group, each sample obtained by pooling lymph nodes from 2 mice). B. % of lymphoid cells co-expressing CD4 and TcRβ (n = 3–4). C. % CD4⁻TcRβ⁺ cells (n = 3–4). D. % cells lacking TcRβ (n = 3–4). E. Proliferation assay in vitro for culture medium (negative control), the inciting antigen (MOG_35–55) and co-stimulation with T cell expander beads (CD3/CD28); n = 3–4 samples (each sample pooled from 2 mice) for each group. Representative flow cytometry plots are shown in right side panels for indicated groups. X-axis shows Oregon Green fluorescence, y-axis shows CD4 labeling. Gating for CD4⁺ cells is outlined in blue, while gating for proliferating cells is outlined in purple.