Priming of <i>pik3cg^−/−</i> CD4⁺ T cells is reduced during EAE.

<p>(<b>A</b>) Frequencies of CD4⁺ cells in draining LN that are CD69⁺ on day 9 post-immunisation for EAE as determined by flow cytometry. A representative histogram overlay gating on CD4⁺ cells is shown (filled = isotype control on WT, solid line = anti-CD69 on WT, dotted line = anti-CD69 on <i>pik3cg^−/−</i>) (<b>B</b>) <i>In vivo</i> proliferation of CD4⁺ cells, measured by BrdU incorporation, is reduced in <i>pik3cg</i>^−/− mice at day 9 post-immunization for EAE. (n = 6 mice per group). Representative dot plots gating on CD4⁺ cells are shown. (<b>C</b>) Expression of chemokine receptors by CD4⁺ T cells indicative of T cell activation was determined by flow cytometry. Representative histogram overlays showing expression of CCR7, CCR6 and CXCR3 are shown (filled = isotype control, solid line = WT, dotted line = <i>pik3cg^−/−</i>). (<b>D</b>) Expression of CD62L, CD49d and PSGL-1 by CD4+ cells from spleen of day 9 immunised mice (n = 3 mice per group). All data shown are mean ± s.e.m. (*, p<0.05).