Rapamycin treatment induced a T cells lymphopenia sparing B cells.

<p>A: absolute lymphocyte number in draining lymph nodes, spleen, and non-draining lymph nodes is decreased in rapamycin-treated mice compare to controls in a dose-dependent fashion. B: quantification of the different sub-populations of lymphocytes: B lymphocytes (B, CD19⁺B220⁺), T (CD3⁺), T CD4⁺ (CD3⁺CD4⁺) and T CD8⁺ (CD3⁺CD8⁺) cells. * p<0.05, *** p<0.001. White histogram bars: control mice, black histogram bars: rapamycin treated mice (3 mg/kg/day). C: percent of T or B lymphocytes in draining lymph nodes. D: Representative dot plot of flow cytometry analysis of draining lymph nodes for percentage of pre-activated CD4⁺ T cell (CD3⁺CD4⁺FoxP3⁻CD69⁺) and percentage of pre-activated CD4⁺ T cell (CD3⁺CD4⁺FoxP3⁻CD69⁺) in controls and rapamycin (3 mg/kg/day) treated mice.