PMFs calculated from the umbrella sampling, coarse-grained simulations for each of the different interfaces: MOPr TM1,2,H8/TM1,2,H8 is shown in red (error bars in pink), MOPr TM5,6/TM5,6 is shown in blue (error bars in light blue), and KOPr TM1,2,H8/TM1,2,H8 is in green (error bars in light green).

<p>The grayed region denoted ‘monomer’ is that in which the PMF curves were aligned to free-energy = 0. The dashed lines, shown at r = 3.12 nm, 3.64 nm, 3.80 nm, 4.00 nm, 4.60 nm, and 5.40 nm, indicate the values of separation at which the inter-protomer contacts were assessed.