Treatment with 1-ButOH alters the morphology of the Golgi apparatus.

<p>A) Cells were untreated or treated with 0.5% 1-ButOH or 5 µg/mL BFA for the indicated times and the Golgi apparatus immunostained for GM-130. With treatment with 1-ButOH, the Golgi apparatus becomes disorganized and the saccules are dilated, while the treatment with BFA resulted in a vesiculation and spreading of the Golgi apparatus. Images are Z-series projections. Bar, 16 µm. B) Quantification of the Golgi volume by pixel intensity of GM-130 staining-thresholded areas. After 15 min of incubation with 1-ButOH there is a significant increase in the volume of the Golgi apparatus (p<0.05, Dunnett’s Multiple Comparison Test). C) Ultrastructural analysis shows control untreated cells with a characteristic Golgi apparatus composed of stacks of flattened cisternae. In cells treated with 0.5% 1-ButOH for 5 min the Golgi saccules are slightly swollen and by 15 min the Golgi apparatus is disorganized and the saccules are dilated. Numerous coated vesicles (arrows) are evident in close proximity to the swollen cisternae after 5 min of treatment with 0.5% 1-ButOH. Treatment with 0.5% <i>tert-</i>ButOH for 5 min or 15 min does not modify the structure of Golgi apparatus. (G, Golgi apparatus). Bar, 500 nm.