Golgi stress induced Golgi disassembly in HSP47 siRNA-transfected NIH3T3 cells.

<p>Representative electron micrographs of NIH3T3 cells. (A) Electron micrographs of NIH3T3 cells at 2 d after transfection with scrambled or HSP47 siRNAs and 3 d after treatment with DMSO or GalNAc-bn. GalNAc-bn treatment induced numerous vacuoles around the Golgi apparatus. Cont, untransfected cells; Scr, scrambled siRNA-transfected cells; siRNA, HSP47 siRNA-transfected cells. N, nucleus; g, Golgi apparatus; m, mitochondria; c, primary cilium. Scale bar: 4 µm. (B) Electron micrographs of NIH3T3 cells at 2 d after transfection with HSP47 siRNA and 3 d after treatment with GalNAc-bn. There are 3 nuclei in 1 cell and numerous vacuoles extending into the cytoplasm. N, nucleus. Scale bar: 20 µm. (C) Selection of high magnification images of the small rectangles in panel C. Numerous vacuoles were observed not only around the Golgi apparatus but also in the cytoplasm. g, Golgi apparatus; m, mitochondria. Scale bar: 4 µm.