Changes in morphology of Golgi apparatus (GA) and distribution of ceramide (Cer) in A375 upon incubation with R-DIM-P-LF11-322 and DIM-LF11-318.

Overlay images of melanoma cells (A375) with stained Golgi apparatus (GA), respectively ceramide (red, BODIPYceramide) and nucleus (blue, NucBlue R37606) in presence of 10 μM peptide R-DIM-P-LF11-322 (left) or DIM-LF11-318 (right) at different incubation periods of 0, 30, 60 and 120 min. R-DIM-P-LF11-322 causes distribution of the Golgi marker BODIPY ceramide into the cytosol and slight formation of domains in the plasma membrane (arrows) within 120 min indicating a strong effect on the Golgi apparatus and the distribution of ceramide. DIM-LF11-318 already induces strong staining of the cytosol and plasma membrane after 30 min, indicating even higher stress on the Golgi and formation of punctual domains of ceramide (arrows) in the plasma membrane. Bottom OL BF: Overlay of bright field (OL BF) and respective fluorescence. Arrows indicate membrane debris upon incubation with DIM-LF11-318. Pictures are representative for a series of three experiments.