Chromosome dynamics during DNA resection in single yeast cells.

<p><b>A</b>: Time-lapse fluorescence microscopy showing ParB1::mCherry-INT1 and ParB2::GFP-INT2 foci after induction of HO synthesis. Both signals persisted during acquisition periods of >60 min (>1500 exposures), with minimal bleaching. Representative single plane images are shown. <b>B</b>: Growth on YPE-D and YPE-Gal of wt and mutant strains bearing or not pGal-HO or ParB expressing plasmids as indicated. Cells were incubated 24 h or 48 h at 30°C and plated in 10× dilution increments. <b>C</b>: Fluorescence intensity quantification during resection in representative cells of wt, <i>yku70</i> and <i>exo1</i>strains. Intensity ratios are calculated relative to adjacent background levels. Background bleaches rapidly during initial acquisition, increasing the signal ratio. <b>D</b>: Time course of resection in wt and <i>yku70</i> cells, measured as percentage of fluorescent INT1 and INT2 foci newly disappeared at each time point. No INT2 resection was detected in wt cells within the time of the experiment.