Time lapse microscopy of chromatin dynamics during resection.

<p>2D stacks (50×200 ms frames) of the ParB2-GFP focus were acquired in wt and <i>yku</i> strains in which the ParB1-mCherry spot was lost, starting at 8 minutes post-addition of galactose to the medium. <b>A</b>. Mean-square displacement (MSD) for wt (n = 12) and <i>yku</i> (n = 3) cells of the uncleaved locus (black line) and at the indicated times after HO induction. <b>B</b>. Diffusion coefficients (D) for wt (black) and <i>yku</i> (red) calculated from the slope of the first 2 sec of the MSD shown in A and normalized to the average D = 1.9×10-2 µm2/sec for uncut DNA set at 100%.