Gene expression states in PEV strains assessed by time-lapse imaging of single yeast cells.

<p>(A) Schematic illustration of the yeast strains expressing EGFP and mCherry. Time-lapse images of the Euchromatin/Euchromatin (FUY257) (B) and TEL-VR PEV/Euchromatin (FUY355) (C) strains expressing <i>HTB1-EGFP</i> (green fluorescence) and <i>HTB1-2x mCherry</i> (red fluorescence). Overlap of the two fluorescent markers is indicated by yellow coloring. Image stacks were taken at 40 min intervals over a 12 h period and the differential interference contrast (DIC) image was merged. The larger panel shows a wide focus image in which only the <i>HTB1-EGFP</i> signal is displayed. Single cells are shown in the insets at the top right corner of each panel; the red arrowhead indicates the same cell in each image. Scale bar, 10 µm. See also Movies S1 and S2.