Myosin Va is necessary for somatodentritic targeting of EGFP-Stau1.

<p><b>A.</b> Following expression in a cortical neuron in dissociated culture EGFP-Stau1 is present in the soma and dendrites, but not in the axon. <b>B</b>. Co-expressed HA-mCherry localizes to the axon as well as to the soma and dendrites in the same cell as in (A). <b>C</b>. Merge of HA-mCherry (red) and EGFP-Stau1 (green). <b>D.</b> High power image of boxed region from (<b>C</b>). <b>E</b>. EGFP-Stau1 is present in the axon as well as in the soma and dendrites when co-expressed with HA-dnMyoVa (<b>F</b>), a dominant-negative variant of Myosin Va. Arrowheads point to the axon. Scale bars represent 10 μm. <b>G</b>. Merge of HA-dnMyoVa (red) and EGFP-Stau1 (green). <b>H</b>. High power image of boxed region from (G). <b>I</b>. A straightened axon from the neuron in (A-D) co-expressing HA-mCherry (upper panel) shows a relative absence of EGFP-Stau1 staining. In contrast, a straightened axon from the neuron in (E-H) expressing HA-dnMyoVa (lower panel) shows EGFP-Stau1 staining. <b>J</b>. EGFP-Stau1 staining vs. distance in the distal axon for the neuron in (A-D; red) and the neuron in (E-H; blue).