Intact actin filaments are necessary for somatodendritic localization of EGFP-Stau1.

<p><b>A</b>. Following exposure to Cytochalasin D (4 μM) for 3 hours EGFP-Stau1 is present in the axon as well as in the dendritic compartment. <b>B</b> HA-mCherry staining in the same cell as in A. <b>C</b>. Merge of EGFP-Stau1 (green) and HA-mCherry (red). <b>D</b>. High power image of boxed area in (C). <b>E</b>. EGFP-Stau1 is absent from the axonal compartment of cortical neurons expressing HA-mCherry (<b>F</b>) and exposed to DMSO. <b>G</b>. Merge of EGFP-Stau1 (green) and HA-mCherry (red). <b>H</b>. High power image of boxed area in (G). <b>I</b>. EGFP-Stau1 axon to dendrite ratio is significantly higher in cells exposed to Cytochalasin D than in cells exposed only to DMSO. * indicates p<0.03 (Wilcoxon). Scale bar 10 μm.