<p>The pGL3-hTERT-Luc, pGL3-Basic or pGL3-Control plasmids were co-transfected with pRL-SV40 plasmid into each cell line. The relative luciferase activity was determined. All data of firefly luciferase activity were corrected for renilla luciferase activity to account for variation in transfection efficiency and expressed as a percentage of results using the positive control. Each transfection was conducted in triplicate, and data represent the mean±sd.</p
<p>WT −365 hP2 or −340/−315 hP2 constructs were co-transfected with an empty vector (pcDNA3) or a pl...
<p>Luciferase assays were used to determine the promoter activities of the luciferase expressing pla...
<p>For each time point, measured in days from the received time of individual clones covering a 60 m...
<p>The luciferase reporter plasmids containing the <i>hTERT</i> gene promoter region with rs2853669T...
<p>The promoter region delineated by FP and RP primers (as shown in <a href="http://www.plosone.org/...
<p>Cells were transfected with the indicated siRNA and a plasmid containing firefly luciferase under...
<p>G-418 resistant cell clones were seeded in 24-well tissue culture plates at 50–70% density in DME...
<p>Four consecutive <i>hTFF3</i> promoter mutants were transfected into HEK 293 and LS-174T cells. T...
<p>PBMC were transfected with C- or T-bearing reporter vectors with or without pretreatment of over-...
<p>HeLa cells transfected with pGL3-3'UTR-RARB and pcDNA3-miR-146a-5p (A) or pcDNA3-miR-146b-5p (B)....
<p><i>hTFF3</i> promoters with different lengths were transfected into HEK 293 and LS-174T cells. Th...
<p>(HeLa cells were transfected with the pRL-TK containing <i>Renilla</i> luciferase gene and the in...
<p>The 5′-flanking region of the <i>HIBADH</i> gene containing T or C loci (designated as pGL3-T and...
<p>(A) Luciferase reporter constructs with different inserts were transiently transfected in HEK293 ...
<p>Activity of firefly luciferase expressed from the reporter vector was normalized with <i>Renilla<...
<p>WT −365 hP2 or −340/−315 hP2 constructs were co-transfected with an empty vector (pcDNA3) or a pl...
<p>Luciferase assays were used to determine the promoter activities of the luciferase expressing pla...
<p>For each time point, measured in days from the received time of individual clones covering a 60 m...
<p>The luciferase reporter plasmids containing the <i>hTERT</i> gene promoter region with rs2853669T...
<p>The promoter region delineated by FP and RP primers (as shown in <a href="http://www.plosone.org/...
<p>Cells were transfected with the indicated siRNA and a plasmid containing firefly luciferase under...
<p>G-418 resistant cell clones were seeded in 24-well tissue culture plates at 50–70% density in DME...
<p>Four consecutive <i>hTFF3</i> promoter mutants were transfected into HEK 293 and LS-174T cells. T...
<p>PBMC were transfected with C- or T-bearing reporter vectors with or without pretreatment of over-...
<p>HeLa cells transfected with pGL3-3'UTR-RARB and pcDNA3-miR-146a-5p (A) or pcDNA3-miR-146b-5p (B)....
<p><i>hTFF3</i> promoters with different lengths were transfected into HEK 293 and LS-174T cells. Th...
<p>(HeLa cells were transfected with the pRL-TK containing <i>Renilla</i> luciferase gene and the in...
<p>The 5′-flanking region of the <i>HIBADH</i> gene containing T or C loci (designated as pGL3-T and...
<p>(A) Luciferase reporter constructs with different inserts were transiently transfected in HEK293 ...
<p>Activity of firefly luciferase expressed from the reporter vector was normalized with <i>Renilla<...
<p>WT −365 hP2 or −340/−315 hP2 constructs were co-transfected with an empty vector (pcDNA3) or a pl...
<p>Luciferase assays were used to determine the promoter activities of the luciferase expressing pla...
<p>For each time point, measured in days from the received time of individual clones covering a 60 m...