Assessment of BaP metabolites in A549 cells using inhibitors of glucuronidation and sulfation.

<p>A) Measurement of 3OH metabolite in A549 cells supplemented with BSA-conjugated fatty acids (LA, DHA, BSA carrier control) for 24 h followed by 2 µM BaP for 24 h in the presence or absence of 500 units of β-glucuronidase. B) Measurement of t7,8 metabolite in A549 cells treated with BSA-conjugated fatty acids (LA, DHA, BSA carrier control) for 24 h followed by 2 µM BaP for 24 h in the presence or absence of 40 µM triclosan. Data represents mean normalized intensity ± SEM of at least 30 images per treatment and 30 cells per image. * indicates significant difference from the corresponding control using two-tailed t-test at p<0.05. Note that glucuronidation (indicated by increase in 3OH due to the presence of β-glucuronidase) is not significant in DHA or LA treated cells while sulfation (indicated by increase in t7,8 due to the presence of triclosan) is significantly enhanced in DHA and LA when compared to BSA.