Analysis of migration and invasion of CpG-ODN treated HB cells.

<p>(<b>A</b>) Evaluation of cell migration by scratch wound healing assay: Cells were allowed to migrate with and without 0.8 μM CpG-ODN. Cell migration into the wound surface was then monitored by microscopy after 24 h of the CpG-ODN treatment and reported as the width of remaining wounded area relative to the initial wound area. (<b>B</b>)Evaluation of cell invasion by transwell assay. Representative photomicrographs of the membrane-associated cells (jacinth part) were assayed by Eosin staining (×400). (<b>C</b>)Graphical representation of scratch wound assay. The average distance of cell invasion was significantly greater in the CpG-ODN treated sample. **P<0.01. (<b>D</b>)Semi-quantitative analysis of the stimulate-invasion effects of CpG-ODN on HB cells. “% of control” denotes the mean number of the cells expressed as a proportion of the control group and the average of three independent experiments ± S.D. **p<0.01, compared with the lane 2; ^★p<0.05, compared with the lane 3. (<b>E</b>)Colorimetric analysis of CpG-ODN modulation of HB cell invasiveness with different concentrations (0∼0.8 μM) for 48 h by the CytoSelect cell invasion assay. *p<0.05; **p<0.01, compared with lane 1.