OSM-mediated wound healing in HCT116 cells is MEK-1- and STAT3-dependent.

<p>Wounding assays were performed to analyze cell migration. Presented are representative images of unstimulated HCT116 cells at baseline (left panel) and after 16 hours (right panel) (A) and OSM-stimulated cells at baseline (left panel) and after 16 hours (right panel) (B). (C) OSM (100 ng/mL) induced a significant increase of cell migration (* p = 3.9×10⁻⁵ vs. unstimulated controls). Preincubation with the MEK-1 inhibitor PD98059 (10 µM) and STAT3 siRNA (5 µM) reduced OSM-induced cell migration in the wounding assay (** p = 1.7×10⁻⁵ and ^# p = 0.01 vs. OSM-stimulated cells). In all experiments, relative cell overgrowth in unstimulated control cells was arbitrarily set to 1.0.