Cellular and humoral immune responses by splenocytes of BALB/c mice after booster immunization.

<p>A) Cytokine production from the splenocytes of 4 mice in the designated groups 3 weeks after last immunization. Single cell suspensions were cultured in duplicate in the presence of recombinant CPA/CPB or freeze-thawed recombinant <i>L. tarentolae</i>. Culture supernatants were assayed for IFN-γ, IL-4, IL-2 and TNF-α production by ELISA. Each bar represents the Mean±SD of 4 mice per group. B) Antigen specific antibody responses against <i>L. tarentolae CPA/CPB/EGFP</i> and SGH before parasite challenge. Data were generated as Mean±SD for sera of individual mice within their respective vaccination group (n = 8–10). The asterisk indicates the significant difference between values at the indicated time points as determined by Mann Whitney U test (<i>p</i><0.05 denoted as *), compared to the control group (G4). Results are representative of two independent experiments.