The interaction between FHL3 and MT-1X <i>in vivo</i>.

<p>293T cells were transfected by Myc-MT-1X with HA-FHL3. Lane 1: lysate of cells transfected with Myc-MT-1X and HA-FHL3. 2: IP of cells with anti-IgG monoclonal antibody as negative control. 3: IP of cells with anti-Myc monoclonal antibody; After 30 h, total cell lysates were analyzed before co-immunoprecipitation to verify expression of Myc-MT-1X and HA-FHL3 (lane 1). After co-immunoprecipitation with anti-Myc tag antibody, MT-1X was detected only in the presence of immune complexes of Myc-FHL3 (lane 3).