MLK3 physically interacted with AMPKα1.

<p>A: HEK293T cells were transfected with GST-MLK3 or GST-LKB1, and then MLK3 and LKB1 were pulled down with GSH beads and blotted with anti-GST antibody. B: HEK293T cells were transfected with GST empty vector, GST-MLK3 or GST-LKB1, and then recombinant proteins were pulled down with GSH beads and blotted with anti-AMPKα, anti-AMPKα1, as well as anti-AMPKα2. C: GST-MLK3 or GST was co-expressed either with Myc-AMPKα1 or Myc-AMPKα2 in HEK293T cells. Immunoblotting was performed on GSH-pulldown and crude extracts with antibodies against GST and Myc, respectively.