Effect of PTH1-34 on osteoblastic bone formation in <i>Bmi-1^-/-</i> mice.

<p>Representative micrographs of paraffin-embedded sections for tibial metaphyseal regions from 4-week old vehicle-treated wild-type (WT) and <i>Bmi-1^-/-</i> mice (KO) and PTH1-34 treated <i>Bmi-1^-/-</i> mice (KO+PTH) stained (A) histologically with hematoxylin & eosin (HE, ×400), (C) histochemically for alkaline phosphatase (ALP, ×200), immunohistochemically for (E) type I collagen (ColI, ×200), (G) osteopontin (OPN, ×200), (I) osterix (x400) and (K) PTHR (x400). (B) Osteoblast counts (#/mm²), (D) ALP-positive areas, (F) type I collagen- or (H) OPN- or (J) osterix- or (L) PTHR-immunopositive areas were measured by computer-assisted image analysis. For each genotype, N = 6; *: <i>p</i><0.05, **: <i>p</i><0.01, ***: <i>p</i><0.001, compared to vehicle-treated WT mice; #: <i>p</i><0.05, ##: <i>p</i><0.01, ###: <i>p</i><0.001 compared to vehicle-treated <i>Bmi-1^-/-</i> mice.