Effect of PTH1-34 on the length of long bones and trabecular bone volume in <i>Bmi-1^-/-</i> mice.

<p>Representative radiographs, (B) quantitation of the length of tibiae, (C) 3-dimensional reconstructed longitudinal sections of micro-CT scanning images and (D) micrographs of paraffin sections of the tibiae stained with Siries Red for total collagen from 4-week-old vehicle-treated wild-type (WT) and <i>Bmi-1^-/-</i> mice (KO) and PTH1-34-treated <i>Bmi-1^-/-</i> mice (KO+PTH), magnification, ×50. (E) Quantitation of trabecular bone volume relative to tissue volume (BV/TV, %) in metaphyseal regions. For each genotype, n = 6; *: <i>p</i><0.05, **: <i>p</i><0.01, ***: <i>p</i><0.001, compared to vehicle-treated WT mice; ###: <i>p</i><0.001 compared to vehicle-treated <i>Bmi-1^-/-</i> mice.