Ca²⁺ transients persist during H1152-induced inhibition of phasic contractions in mesenteric collecting lymphatics.

<p><i>A.</i> Kymograph of the lymphatic shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094082#pone.0094082.s006" target="_blank">Movie S3</a>, with the x-axis representing time, the y-axis representing distance, and the heat map representing the intensity of the 340/380 ratio. <i>B</i>–<i>C.</i> Changes in the 340/380 ratio and lymphatic diameter versus time. <i>D.</i> Kymograph of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0094082#pone.0094082.s007" target="_blank">Movie S4</a>, immediately after H1152 was added to the bath at a final concentration of 10 μM. <i>E</i>–<i>F</i> Changes in 340/380 ratio and lymphatic diameter versus time immediately after H1152 was added. <i>G</i>. Time course of the mean changes in CF and transient frequency (TF) in response to H1152. <i>H</i>. Time course of mean changes in AMP/MaxD and the Ca²⁺ transient magnitude (Δ340/380) after H1152 treatment. <i>I</i>. Time course of mean changes in EDD/MaxD and the basal (diastolic) 340/380 ratio between Ca²⁺ transients in response to H1152. *P<0.05 and **P<0.01 versus baseline. †P<0.05, CF vs. TF, same time point. N = 5 lymphatic vessels studied.