mEPSC amplitude and frequency change in Hu97/18 SPNs.

<p>Cells were patch-clamped with potassium-based internal solution at V_h = -70 mV and the initial sEPSCs were recorded. Then, TTX was added to the external solution which allowed for the extraction and analysis of mEPSCs. (A) Representative mEPSC traces for Hu18/18 (left) and Hu97/18 SPNs (right). (B) The average mEPSC amplitude of Hu18/18 and Hu97/18 cells (p = 0.1). (C) Cumulative probability showed a decrease in sEPSC amplitude in Hu97/18 SPNs (significant genotype and amplitude interaction, p<0.0001). (D) mEPSC amplitude presented as a percentage of the initial sEPSC amplitude. There was no difference between Hu18/18 and Hu97/18 SPNs. (E) The mean mEPSC frequency trended towards a decrease in Hu97/18 SPNs but did not reach statistical significance (p = 0.052). (F) Cumulative probability showed an increase in mEPSC inter-event intervals in Hu97/18 SPNs (significant genotype and inter-event intervals interaction, p<0.0001). (G) mEPSC frequency presented as a percentage of the initial sEPSC frequency. There was no difference between Hu18/18 and Hu97/18 SPNs. For all experiments, Hu18/18 n = 9 and Hu97/18 n = 5.