In vivo imaging of BoHV-4-A-CMVlucΔTK inoculate mice.

<p>A) Representative images of groups of mice (n = 3 per group) intra-peritoneal (A and blue line in C) or intra-venous (B and red line in C) inoculated with BoHV-4-A-CMVlucΔTK. Mice were monitored at 1, 2, 3, 7 and 14 (not shown) days post inoculation by BLI; light intensity was quantified using the LivingImage software (C). The experiment was repeated three times and each point represents the mean ± standard deviation of 9 animals. Data were expressed as photons/second (S)/cm² and statistical differences were tested by One Way ANOVA followed by Dunnet’s post hoc test for group comparisons. Results are reported as mean ± SD and significance attributed when <i>P</i><0.05 (*) or <i>P</i><0.01 (**). D) Representative BLI image of explanted abdominal organs [uterus (U; in red), spleen (S; in red), kidney (K; in red) and liver (L; in red)] from BoHV-4-A-CMVlucΔTK intra-peritoneal inoculated mice (n = 3) at 3 days post inoculation. The same experiment was repeated 3 times with identical results.