CD31 expression in Edn2KO and Edn2+/? ovaries after EDN2, vehicle, or no injection.

<p>Frozen sections were made from Edn2+/? or Edn2KO mouse ovaries collected at hCG16. Mice received injection with EDN2 peptide, PBS, or no injection at hCG12. Ovaries were stained with CD31 (PECAM-1) to visualize the vascular network. Note that positive staining was seen inside the CL of heterozygote ovaries, but was not present inside any demarcated structures of Edn2KO ovaries treated with EDN2 or PBS. Arrows indicate vascularization within representative CL of ovaries treated with PBS or no injection.