Lack of CL formation in the Edn2KO ovary following superovulation induction.

<p>Adjacent ovarian sections of the Edn2KO mice and heterozygous littermates collected at hCG24 were stained with αSMA antibody and P450scc antibody. Edn2+/? ovaries display αSMA and P450scc staining within CL, as well as in the interstitium around clearly demarcated follicles. In the Edn2KO ovaries, CL are absent and multiple large Graafian follicles are instead present in the periphery of the ovaries. Some, but not all, contain an oocyte with a partial or complete cumulus layer. Histology was similar between all Edn2KO ovaries regardless of number of oocytes ovulated. CL: corpus lutea.