Root Mean Square Error (RMSE) on the prediction of the effect of destabilizing mutations.

<p>RMSE of the linear regression through the origin between experimental and predicted variations in free energy variations (ΔΔG). Box plots generated from 10000 resampling bootstrapping iterations on the subset of destabilizing mutations (experimental ΔΔG>0.5 kcal/mol) in the PoPMuSiC-2.0 dataset (N = 174). The results for destabilizing mutations mirror to a great extent those for the entire dataset given that they represent 57% of the entire dataset.