Modeling of the wild type (WT) and the mutant (Dβ484N) <i>Ec</i>PA stability at pH 10.0.

<p>Root mean square deviation (RMSD) for each enzyme variant has been averaged over three independent MD runs. The mean and standard deviation are shown in angstroms. N_res – number of amino acid residues in a structural unit. Δ_RMSD = RMSD_Dβ484N – RMSD_WT and <i>f</i> = <i>f</i>(RMSD_Dβ484N ≥ RMSD_WT) were calculated as explained in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100643#s4" target="_blank">Methods</a>, section 2. Lower <i>f</i> values indicate a significant stabilizing effect of the mutation compared to the wild type enzyme while Δ_RMSD estimates the degree of stabilization.