IFN-γ increases autophagosome formation.

<p>4A) Confocal microscopy for non-CF and CF macrophages infected with m-RFP expressing k56-2. IFN-y treatment was administered after 1 hour of infection for a 4 hour treatment period. LC3 is stained green, and macrophage nuclei are stained blue with DAPI. Co-localization of bacteria with LC3 is noted in yellow in the bottom panel as noted by white arrows. 4B) 24 hour IFN-γ and rapamycin treatments similar to 4A. 4C) Summary of scored bacteria per 100 macrophages from individual subjects for the confocal microscopy experiments from 4B, n = 17 subjects, with 2 replicates per subject, unpaired t-test. 4D) The percentage of bacterial co-localization with LC3 was scored for over 100 macrophages per condition from 4B, n = 17 subjects, with 2 replicates per subject, unpaired t-test. 4E) CFU counts for non-CF and CF macrophages infected with MHK1 for 24 hours (n = 6).