Autophagic flux is decreased in CF.

<p>3A) Representative immunoblot of <u>4 </u>hour treatment protein lysates for LC3-1 and LC3-2 from non-CF and CF macrophages, with corresponding summed band densitometries normalized to loading control. All lanes are marked with a “+” if the corresponding treatments were added: <i>B. cenocepacia</i> infection (k56-2), autophagy stimulators (IFN-y, Rapamycin) and autophagy inhibitors (Bafilomycin, 3-MA). Inhibitors were added one hour prior to infection. Autophagy stimulators were added one hour after infection for a total of 4 hours of treatment. Data is representative of 3 independent experiments. 3B) Representative immunoblot of <u>24 hour</u> treatment protein lysates for LC3-1 and LC3-2 from non-CF and CF macrophages with corresponding summed band densitometries normalized to loading control. All lanes are marked with a “+” if the corresponding treatments were added: <i>B. cenocepacia</i> infection (k56-2), autophagy stimulators (IFN-y, Rapamycin) and autophagy inhibitors (Bafilomycin, 3-MA). Inhibitors were added one hour prior to infection. Autophagy stimulators were added one hour after infection for a total of 24 hours of treatment. Data is representative of 3 independent experiments.