Western blot of spleen PrPres in TgOvPrP4 mice infected with classical scrapie or BSE.

<p>Mice were challenged by oral (lane 5–8) or the intra-cerebral route (lanes 1–4). A) Western blot detection of PrPres by Sha 31 antibody of different sources derived from classical BSE in cattle (lanes 1, 5), classical BSE in sheep (lanes 2, 6), C506M3 scrapie strain (lanes 3, 7) and natural classical scrapie isolate (lanes 4, 8). The equivalent tissue quantities loaded per lane are indicated at the bottom of each lane. Bars to the left indicate the 29.0 and 20.1 kDa marker positions. B) Differences of the unglycosylated PrPres apparent molecular masses compared to that in mice infected with source derived from ovine BSE by the same inoculation route (means +/− SD of 5 repeated assays). The raw apparent molecular mass of unglycosylated PrPres is indicated for the ovine BSE infected mouse. C) Tern-plot representation of the proportions of diglycosylated (H), monoglycosylated (M) and unglycosylated (L) bands of PrPres for different sources derived from classical BSE in cattle (□), classical BSE in sheep (▵), C506M3 scrapie strain (X) and natural classical scrapie isolate (+) (blue: intra-cerebral, red: oral). The means for all repetitions (n = 5) are plotted.