Immunoblot analysis of PrPres in scrapie-infected mice.

<p>(A) Panel A shows immunoblots of brain homogenates tested using both 0.5mg and 0.25mg brain equivalents per lane. PrPres levels in clinical Tg330 mice (#255 and #019) were slightly lower than levels seen in clinical C57BL/10 mice. All C57BL/10 and Tg330 mice inoculated with 22L and ME7 scrapie were positive for PrPres by western blot at the time of clinical disease. In panels B and C, lanes 1 and 2 had 0.5 mg brain equivalents and other lanes had 1.0mg. (B) RML (stock 06) inoculations. Of fifteen transgenic mice tested, two (#573 and 778) were positive for PrPres at 451 and 518 days respectively. Four of the 13 PrPres-negative mice are also shown. RML (stock 81) inoculation of 14 mice resulted in none positive for PrPres by immunoblot (not shown). (C) 79A inoculations. Of fourteen transgenic mice tested, two mice (#657 and 470) were positive for PrPres at 439 and 545 dpi respectively. Four of the 12 PrPres-negative mice are also shown.