<p>A) Primer and template sequence. The radioactively labelled primer is either 3’ matched or mismatched depending on the template. B) PAGE of primer extension, P = primer, A = match case template, G = mismatch case template. Mutants and <i>KlenTaq</i> wild-type as indicated.</p
Primer sequences (forward and reverse) used to identify the mutant and non mutant (control) individu...
<p>(A) Relative levels of Po or Pf PCR products. The amounts of Po or Pf PCR products of mixed templ...
<p>Sequences of wild type ILPR-I4 and ILPR-I3, a scrambled sequence, and the mutants used in this st...
<p>(A) primer and (partial) template sequence from the prothrombin factor II gene. The 3’ end of the...
<p><b>A-C.</b> Primer extension using oligomers, SCR21 (A), SCR105 (B) and SCR83 (C) for wild type a...
<p>Primers used for mutant construction, complementation and RT-PCR analyses.</p
<p>Primer sequences used to obtain the transforming constructs and to identify mutants by PCR and So...
<p>(A) Principle of the single-nucleotide primer extension method illustrated through analysis of a ...
<p>Wt: Wild-type; Mut: mutant; F: Forward primer; R: Reverse primer. The sequences of restriction si...
<p>The PCR product from three mutated isolates <i>ssaS</i>::Km (lanes 1–3) and the wild type gene <i...
<p><b>A.</b> Schematic representation of the strategy of primer extension used for detecting pause s...
<p>*<sup>1</sup> WT, wild-type; *<sup>2</sup> MUT, mutant.</p><p>Sequence of allele-specific primers...
<p>Features of primer pairs (ΔTm) are counted once, features of single primers (ΔG) were counted sep...
<p>Primers used to detect point mutations by sequence specific PCR, Inner primers end on the indicat...
<p>Nucleotides of the primers that differ from the wild type sequence are presented in bold.</p
Primer sequences (forward and reverse) used to identify the mutant and non mutant (control) individu...
<p>(A) Relative levels of Po or Pf PCR products. The amounts of Po or Pf PCR products of mixed templ...
<p>Sequences of wild type ILPR-I4 and ILPR-I3, a scrambled sequence, and the mutants used in this st...
<p>(A) primer and (partial) template sequence from the prothrombin factor II gene. The 3’ end of the...
<p><b>A-C.</b> Primer extension using oligomers, SCR21 (A), SCR105 (B) and SCR83 (C) for wild type a...
<p>Primers used for mutant construction, complementation and RT-PCR analyses.</p
<p>Primer sequences used to obtain the transforming constructs and to identify mutants by PCR and So...
<p>(A) Principle of the single-nucleotide primer extension method illustrated through analysis of a ...
<p>Wt: Wild-type; Mut: mutant; F: Forward primer; R: Reverse primer. The sequences of restriction si...
<p>The PCR product from three mutated isolates <i>ssaS</i>::Km (lanes 1–3) and the wild type gene <i...
<p><b>A.</b> Schematic representation of the strategy of primer extension used for detecting pause s...
<p>*<sup>1</sup> WT, wild-type; *<sup>2</sup> MUT, mutant.</p><p>Sequence of allele-specific primers...
<p>Features of primer pairs (ΔTm) are counted once, features of single primers (ΔG) were counted sep...
<p>Primers used to detect point mutations by sequence specific PCR, Inner primers end on the indicat...
<p>Nucleotides of the primers that differ from the wild type sequence are presented in bold.</p
Primer sequences (forward and reverse) used to identify the mutant and non mutant (control) individu...
<p>(A) Relative levels of Po or Pf PCR products. The amounts of Po or Pf PCR products of mixed templ...
<p>Sequences of wild type ILPR-I4 and ILPR-I3, a scrambled sequence, and the mutants used in this st...