Single nucleotide primer extension experiments using KOD pol wildtype and mutants.

<p>(A) primer and (partial) template sequence from the prothrombin factor II gene. The 3’ end of the radioactively labeled primer was either matched or mismatched to the template. (B) PAGE analysis of single nucleotide extension of the primer by either KOD pol wildtype or mutants, as indicated. Reactions were carried out for the indicated times. (C) + (D) percentage of primer conversion vs time for match and mismatch case, respectively. Gel bands were quantified with the Quantity One software from BioRad, results are shown as means +/- SD of three independent experiments. Graphs were prepared with Origin2015.