Primer extension on wild type and mutant G-quadruplex motif at <i>BCL2</i> mbr using multiple primers.

<p><b>A-C.</b> Primer extension using oligomers, SCR21 (A), SCR105 (B) and SCR83 (C) for wild type and mutant plasmids. In each case, lane 1 is primer alone, lanes 2–4 are primer extensions at different temperatures as indicated. The pause sites generated as a result of replication blocks due to G-quadruplex formation are marked with square brackets. “WT” denotes wild type and “MT” denotes mutant plasmids. “M” is 50 nt ladder.