eIF4G, but not eIF3h, is sumoylated.
- Jirapas Jongjitwimol (562777)
- Min Feng (141118)
- Lihong Zhou (106007)
- Oliver Wilkinson (562778)
- Lauren Small (562779)
- Robert Baldock (562780)
- Deborah L. Taylor (562781)
- Duncan Smith (562782)
- Lucas D. Bowler (562783)
- Simon J. Morley (175882)
- Felicity Z. Watts (240991)
Publication date
May 2014
DOIAbstract
<p>His-tagged SUMO was expressed in cells containing genomically tagged (HA) copies of eIF4G (A) and eIF3h (B). WCE = whole cell extract, PD = Ni<sup>2+</sup>-agarose pull down. Blots were probed with anti-HA or anti-SUMO antisera. C. Western blot of whole cell extracts from cells containing genomically tagged eIF4G-HA. UT = untreated, C, K = incubated for 30 min with 100 µg/ml CHX (C) or 1 M KCl (K).</p
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