The effect of SUMOylation on dimerization of EHD3.

<p><b>A</b>. Lysates of HEK293T cells, transiently transfected with different combinations of EHD3 variants, were coimmunoprecipitated with anti-myc antibody. The immunoprecipitates and 5% of the cell lysates were subjected to SDS-PAGE and the corresponding blots were interacted with anti-myc and anti-GFP antibodies. IP: immunoprecipitation; WB: western blot. <b>B.</b> COS-7 cells were transiently cotransfected with plasmids expressing myc-EHD3 together with either GFP-EHD3 or its SUMOylation mutants [EHD3K315R, EHD3K511R, EHD3K(315+511)R]. Twenty-four hours later cells were fixed with 4% paraformaldehyde and visualized (left panel). Right panels depict enlarged regions of the cells. Scale bars represent 10 μm.