<p>CHO cells were cotransfected with plasmids expressing EGFP-fused SOD1 (either wild-type or mutant), HA-tagged SUMO 1/3, and myc-tagged Ubc9. The cells were fixed after 24 h of transfection and immunostained with anti-HA antibody and DyLight594-conjugated anti-mouse IgG antibody, followed by counterstaining with DAPI. Representative images from two independent experiments are shown. The bar in the upper left panel indicates 10 µm. Arrowheads and arrows indicate cells with and without intracellular SOD1 protein aggregates, respectively. In all triple transfections, all GFP-positive cells were DyLight594 (SUMO)-positive (detail in Supporting Information <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0101080#pone.01010...
CHO cells were transfected with plasmids for each SOD1:YFP variant and harvested at 24 hours for imm...
Multiple cellular functions are compromised in amyotrophic lateral sclerosis (ALS). In familial ALS ...
<p>Fluorescent microscopy images of non-transfected and sumo1-YFP transfected <i>Lmna</i><sup>+/+</s...
<p>NSC34 cells were cotransfected with plasmids expressing FLAG-tagged SOD1 (either wild-type or mut...
<p>CHO cells were cotransfected with plasmids expressing EGFP-fused SOD1 (either wild-type or mutant...
<p>NSC34 cells were cotransfected with plasmids expressing FLAG-tagged G93R-SOD1, HA-tagged SUMO1, S...
Mutations in superoxide dismutase 1 (SOD1) are a major cause of familial amyotrophic lateral scleros...
<div><p>Mutations in superoxide dismutase 1 (SOD1) are a major cause of familial amyotrophic lateral...
Mutations in superoxide dismutase 1 (SOD1) are a major cause of familial amyotrophic lateral scleros...
<p><b>A</b>. Lysates of HEK293T cells, transiently transfected with different combinations of EHD3 v...
<p>CHO cells were transiently transfected with vectors to expression WT and ALS-associated variants ...
<p><b>A</b>. Lysates of HEK293T cells, transiently cotransfected with GFP-EHD1 and either wt or one ...
<br>Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the se...
<p><b>A.</b> Lysates of HEK293T cells, transiently transfected with myc–EHD3 or its SUMOylation muta...
<div><p>Background</p><p>By mechanisms yet to be discerned, the co-expression of high levels of wild...
CHO cells were transfected with plasmids for each SOD1:YFP variant and harvested at 24 hours for imm...
Multiple cellular functions are compromised in amyotrophic lateral sclerosis (ALS). In familial ALS ...
<p>Fluorescent microscopy images of non-transfected and sumo1-YFP transfected <i>Lmna</i><sup>+/+</s...
<p>NSC34 cells were cotransfected with plasmids expressing FLAG-tagged SOD1 (either wild-type or mut...
<p>CHO cells were cotransfected with plasmids expressing EGFP-fused SOD1 (either wild-type or mutant...
<p>NSC34 cells were cotransfected with plasmids expressing FLAG-tagged G93R-SOD1, HA-tagged SUMO1, S...
Mutations in superoxide dismutase 1 (SOD1) are a major cause of familial amyotrophic lateral scleros...
<div><p>Mutations in superoxide dismutase 1 (SOD1) are a major cause of familial amyotrophic lateral...
Mutations in superoxide dismutase 1 (SOD1) are a major cause of familial amyotrophic lateral scleros...
<p><b>A</b>. Lysates of HEK293T cells, transiently transfected with different combinations of EHD3 v...
<p>CHO cells were transiently transfected with vectors to expression WT and ALS-associated variants ...
<p><b>A</b>. Lysates of HEK293T cells, transiently cotransfected with GFP-EHD1 and either wt or one ...
<br>Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the se...
<p><b>A.</b> Lysates of HEK293T cells, transiently transfected with myc–EHD3 or its SUMOylation muta...
<div><p>Background</p><p>By mechanisms yet to be discerned, the co-expression of high levels of wild...
CHO cells were transfected with plasmids for each SOD1:YFP variant and harvested at 24 hours for imm...
Multiple cellular functions are compromised in amyotrophic lateral sclerosis (ALS). In familial ALS ...
<p>Fluorescent microscopy images of non-transfected and sumo1-YFP transfected <i>Lmna</i><sup>+/+</s...