Netrin signaling and <i>mig-14/wntless</i> have redundant roles in A/P and D/V guidance of the DTCs.

<p>(A, B) Quantification of phase 3 A/P polarity reversals in single mutants of <i>mig-14/wntless</i>, <i>unc-5</i>, <i>unc-40</i>, <i>unc-6</i> and <i>sfrp-1</i> versus double mutants of <i>mig-14</i> with <i>unc-5</i>, <i>unc-40</i>, or <i>unc-6</i> and of <i>sfrp-1</i> with <i>unc-5(RNAi)</i>. Bars represent the percentage of phase 3 polarity reversals (black) and ‘no turn’ (red) phenotypes. The latter represent a subset of the phase 3 reversals. The corresponding raw data for all panels are presented in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004381#pgen.1004381.s007" target="_blank">Table S2</a>. All strains were analyzed in the background of <i>dnIs31[gly-18p::gfp]</i> to visualize the DTCs. <i>mig-14(ga62); unc-5(ev489)</i> was also analyzed without <i>gly-18p::gfp</i>, which made no difference (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004381#pgen.1004381.s007" target="_blank">Table S2</a>). Most of the progeny of the <i>mig-14(k124); unc-6(ev400)</i> double mutant were not viable, therefore, escapers were analyzed. Error bars indicate standard error of the sample proportion. Comparisons of the total phase 3 A/P reversals for double deficits were made to <i>mig-14(ga62)</i>, <i>mig-14(k124)</i>, or <i>sfrp-1(gk554)</i>. ***P<0.00001 **P<0.001; *P<0.05; ns = not significant (P≥0.05). (C, D) Quantification of the phase 2 D/V migration failures in the strains presented in panels (A) and (B), respectively. Bars represent the percentage of ‘ventralized’ (grey) and ‘no turn’ (red) gonad arms. The latter represent a subset of the phase 2 migration failures. The corresponding raw data for all panels are presented in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004381#pgen.1004381.s007" target="_blank">Table S2</a>. Error bars indicate standard error of the sample proportion. Comparisons of the total phase 2 migration failures (‘no turn’ plus ‘ventralized’ gonad arms) were made between paired single and double deficits. ***P<0.00001 **P<0.001; *P<0.05; ns = not significant.