Netrin and Wnt signaling components function redundantly to regulate A/P axon guidance of the CAN neuron.

<p>Fluorescence micrographs of the indicated mutant strains bearing <i>gly-18::gfp</i> to visualize the CAN neuron. Dorsal is up and anterior is left. Arrows mark the CAN axon trajectories. Asterisk marks the CAN cell body. (A) <i>egl-20(n585)</i> and (B) <i>unc-5(ev489)</i> both display bipolar axon extensions along the A/P axis as in the wild-type. (C) <i>unc-5(e53) egl-20(n585)</i> as well as (D) <i>unc-5(ev489) egl-20(n585)</i> display axon polarity reversals, as well as axon branching and premature terminations (E,F) All of these defects occur predominantly on the posterior side. (G) Quantification of the CAN axon defects in <i>egl-20</i>, <i>unc-5</i>, <i>mig-14/wntless</i> and <i>unc-6</i> single mutants compared to <i>unc-5 egl-20</i> or <i>mig-14; unc-6</i> double mutants, respectively. <i>egl-20(n585)</i> is reportedly temperature sensitive <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004381#pgen.1004381-Whangbo2" target="_blank">[33]</a> hence the analysis was carried out at both 20°C and 25°C. Bars represent the percentage of CAN axon reversals (red), branching (grey), and premature terminations (yellow). The corresponding raw data are presented in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004381#pgen.1004381.s011" target="_blank">Table S6</a>. Error bars indicate standard error of the sample proportion. Comparisons were made to the corresponding single mutant controls. ***P<0.00001 **P<0.001.