Wnt and Netrin signaling components function redundantly to regulate D/V axon guidance of AVM and PVM neurons.

<p>(A) <i>In egl-20(n585)</i> mutants, the AVM neuron sends an axon (arrow) ventrally as in the wild type. (B) <i>unc-5(e53) egl-20(n585)</i> double mutants frequently fail to execute the normal D/V axon migration and the AVM axon migrates longitudinally instead. (C) Quantification of the AVM or PVM axon defects in <i>egl-20</i> and <i>unc-5</i> single mutant compared to <i>unc-5 egl-20</i> double mutant animals or the <i>unc-5 egl-20</i> compared to two independent lines of this double mutant carrying the <i>mec-7::unc-5</i> transgene. Bars represent the percentage of longitudinal axons (red) or partially longitudinal axons (grey). A/P polarity defects including axon reversals (black) and bipolar axons (yellow) were also observed. (D) Quantification of the AVM (black) or PVM (grey) axon defects in <i>mig-14/wntless</i> or <i>unc-6</i> single mutants compared to <i>mig-14; unc-6</i> double mutants. The corresponding raw data are presented in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004381#pgen.1004381.s012" target="_blank">Table S7</a>. Error bars indicate standard error of the sample proportion. Comparisons were made to the corresponding single mutant controls. ***P<0.00001; *P<0.05; ns = not significant.